New Publication: Quantifying 3D Morphology & RNA from Individual Embryos

Iodine-enhanced CT scan projection of a mouse embryo (E11.5) in 3D (top) and an exemplar embryo RNA integrity analysis after staining and scanning, showing 18S and 26S bands as well as a lower marker (bottom).

“Here we present a method that generates high quality iodine contrasted CT images from embryonic soft tissue. This method has two primary advantages over previous methodologies. First, it allows extraction of high quality nucleic acids following the CT scan, generating a 1:1 correspondence between scan and gene expression or DNA profile. Second, it minimizes the shrinkage artifacts associated with fixation and contrasting. The ability to relate a single sample to its genetic profile will allow study of both partially penetrant models and also increase our understanding of how genotype and phenotype are directly related.”

– Lead author, Rebecca Green (Hallgrímsson Lab)

Go read more at Developmental Dynamics!

New Publication: Three-dimensional visualisation of the internal anatomy of the sparrowhawk (Accipiter nisus) forelimb using contrast-enhanced micro-computed tomography

“A three-dimensional model of the wing musculature of the sparrowhawk (Accipiter nisus) was built using μCT and a 3% iodine-buffered formalin solution to test the ability of the technique for visualising wing musculature and obtaining quantitative data of muscle geometry.  This model allows the identification of the individual muscles comprising the avian wing and can be useful for further biomechanical analysis of flight.”

– Lead Author, Fernanda Bribiesca-Contreras

Go read more about the project at PeerJ!

Transverse μCT images of a sparrowhawk (Accipiter nisus) wing (upper panel)—Columns: (A) Control . (B–F) ∼3% (w/v) iodine-buffered formalin solution after three (B), 10 (C), 15 (D), 18 (E), and 25 (F) days. Rows: (G) Corresponds to brachial area, (H) antebrachial area and (I) the manus (scale bar = 5 mm). Three-dimensional model of the wing muscles of a sparrowhawk (lower panel)—three-dimensional model of the wing muscles of a sparrowhawk reconstructed from CT images of the stained wing after 25 days in a ∼3% iodine-buffered formalin solution (superficial muscles are shown in dorsal (left) and ventral (right) view).