New Publication: 3-D mammalian tooth development using diceCT


Histology (a, e), 2D diceCT sections with outlines of layers (b, f) and anterior (c, g) and apical (d, h) views of 3D models of enamel knots developing within upper canine (a–d) and lower dp3 (e–h). Scale bars = 100 μm. (See figure legend in the web version of this manuscript for interpretations of colored regions.)

“We applied the diceCT technique to image, in three dimensions, a mammalian tooth development pattern, using embryos and pouch young of the tammar wallaby (Macropus eugenii). This enabled individual tissue layers within developing teeth to be clearly distinguishable and even allowed us to image single-cell layer tissues with higher magnification sub-volume scans. Within the same scan we could 3D-visualise both the soft and hard tissues present at various stages through tooth development, including the primary and secondary dental laminae, as well as first and second generations of teeth. With these contrast-enhanced scans, we produced 3D models of in-situ tooth development, demonstrating the enormous potential to visualise this and other organogenetic patterns using this technique.”

– Authors Qamariya Nasrullah (@), Marilyn Renfree, & Alistar Evans 

Head over to the Archives of Oral Biology to read the pub and the Evans EvoMorph Lab webpage & Twitter to see more!