New Publication: 3-D mammalian tooth development using diceCT

 

nasrullah5.png
Histology (a, e), 2D diceCT sections with outlines of layers (b, f) and anterior (c, g) and apical (d, h) views of 3D models of enamel knots developing within upper canine (a–d) and lower dp3 (e–h). Scale bars = 100 μm. (See figure legend in the web version of this manuscript for interpretations of colored regions.)

“We applied the diceCT technique to image, in three dimensions, a mammalian tooth development pattern, using embryos and pouch young of the tammar wallaby (Macropus eugenii). This enabled individual tissue layers within developing teeth to be clearly distinguishable and even allowed us to image single-cell layer tissues with higher magnification sub-volume scans. Within the same scan we could 3D-visualise both the soft and hard tissues present at various stages through tooth development, including the primary and secondary dental laminae, as well as first and second generations of teeth. With these contrast-enhanced scans, we produced 3D models of in-situ tooth development, demonstrating the enormous potential to visualise this and other organogenetic patterns using this technique.”

– Authors Qamariya Nasrullah (@), Marilyn Renfree, & Alistar Evans 

Head over to the Archives of Oral Biology to read the pub and the Evans EvoMorph Lab webpage & Twitter to see more!

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out / Change )

Twitter picture

You are commenting using your Twitter account. Log Out / Change )

Facebook photo

You are commenting using your Facebook account. Log Out / Change )

Google+ photo

You are commenting using your Google+ account. Log Out / Change )

Connecting to %s